中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (29): 5428-5432.doi: 10.3969/j.issn. 2095-4344.2012.29. 022

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

丝素蛋白/透明质酸复合多孔支架材料的表征及细胞相容性

范志海1，陆福男2，张 锋3，沈忆新1   

  1. 1苏州大学附属第二医院骨科，江苏省苏州市 215004；
    2甪直人民医院骨科，江苏省苏州市 215127；
    3苏州大学基础医学与生物科学学院，江苏省苏州市 215123
  • 收稿日期:2012-04-09 修回日期:2012-04-21 出版日期:2012-07-15 发布日期:2012-07-15
  • 通讯作者: 沈忆新，教授，硕士生导师，苏州大学附属第二医院骨科，江苏省苏州市 215004 shenyx@mail. szldbz.gov.cn
  • 作者简介:范志海☆，男，1976年生，江西省上饶市人，汉族，苏州大学在读博士，主治医师，主要从事脊柱脊髓的基础与临床研究。 fanzh2006@ 163.com

Characterization and biocompatibility of silk fibroin/hyaluronic acid composite porous scaffolds

Fan Zhi-hai1, Lu Fu-nan2, Zhang Feng3, Shen Yi-xin1   

  1. 1Department of Orthopedics, the Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China;
    2Department of Orthopedics, People’s Hospital of Luzhi, Suzhou 215127, Jiangsu Province, China;
    3School of Basic Medicine and Biology, Soochow University, Suzhou 215123, Jiangsu Province, China
  • Received:2012-04-09 Revised:2012-04-21 Online:2012-07-15 Published:2012-07-15
  • Contact: Shen Yi-xin, Professor, Master’s supervisor, Department of Orthopedics, the Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China shenyx@ mail.szldbz.gov.cn
  • About author:Fan Zhi-hai☆, Studying for doctorate, Attending physician, Department of Orthopedics, the Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China fanzh2006@163.com

PDF

465

被引次数

4

摘要：

背景：丝素蛋白和透明质酸具备细胞外基质两种主要成分特征，并且有良好的生物相容性。
目的：采用冷冻干燥法制备丝素蛋白/透明质酸复合多孔支架，并观察其表征与细胞生物相容性。
方法：将丝素蛋白与透明质酸按10∶1混合通过冷冻干燥法构建丝素蛋白/透明质酸复合多孔支架并观察其表征。将1×108 L-1的第3~5代大鼠骨髓间充质干细胞接种在丝素蛋白/透明质酸复合多孔支架上观察其细胞生物相容性。
结果与结论：采用冷冻干燥法可以成功制备丝素蛋白/透明质酸复合多孔支架，与纯丝素蛋白支架相比，复合支架具有更好的多孔三维结构。此外，支架制备过程中不含有毒溶剂，支持骨髓间充质干细胞的黏附铺展与增殖。说明实验制备的丝素蛋白/透明质酸复合支架的成孔性好，具有良好的细胞生物相容性。

关键词: 丝素蛋白/透明质酸多孔支架, 冷冻干燥, 骨髓间充质干细胞, 细胞相容性, 表征, 生物材料

Abstract:

BACKGROUND: Silk fibroin and hyaluronic acid with good biocompatibility have the features of protein and glycosaminoglycan in the extracellular matrixs.
OBJECTIVE: To prepare silk fibroin/hyaluronic acid composite scaffolds using lyophilization, and to evaluate their characterization and cellular biocompatibility.
METHODS: The silk fibroin/hyaluronic acid composite scaffold was prepared by lyophilization at a ratio of 10:1 and characterized by scanning electron microscope and Fourier Transform infrared spectroscopy. Passages 3-5 bone marrow mesenchymal stem cells from rats at a density of 1×108 cells/L were seeded on the silk fibroin/hyaluronic acid composite scaffold to observe cellular biocompatibility by scanning electron microscope and Laser confocal microscope.
RESULTS AND CONCLUSION: The silk fibroin/hyaluronic acid composite scaffold was successfully prepared by lyophilization, showing better porous three-dimensional structure compared with pure silk fibroin scaffold. Good cell adhesion, spreading and proliferation behavior on the silk fibroin/hyaluronic acid composite scaffolds were observed, indicating that the cellular biocompatibility of the composite scaffolds is preliminarily confirmed.

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